Tarangnan Community Hospital Clinical Laboratory • Clinical Microscopy	Document No.: WI-CM-001-A-V0	CONTROLLED COPY Do not copy or remove
	Parent document: SOP-CM-001, Section 5.2  |  Version: V0
Applies to: Medical Technologists, Phlebotomists, Nursing Staff  |  Effective date: MM-DD-YYYY  |  Supersedes: None

CLEAN-CATCH MIDSTREAM URINE COLLECTION & STABILITY CARD

1. 1
   PATIENT PREPARATIONVerify Patient ID & Explain Procedure: Verify 2 identifiers (Full Name, DOB). Explain midstream clean-catch principles in clear language (Waray-Waray / English). Instruct patient to wash hands thoroughly with soap and clean water. SOP-CM-001 §5.2.1
2. 2
   PERIURETHRAL CLEANSINGPerform Careful Periurethral Cleansing:
   • Female: Spread labia, cleanse front-to-back using clean water/antiseptic wipes; discard wipes. Keep labia parted during voiding.
   • Male: Retract foreskin if uncircumcised, cleanse glans penis thoroughly from meatus outward. SOP-CM-001 §5.2.1
3. 3
   MIDSTREAM VOIDDiscard Initial Stream & Collect Midstream: Allow first stream of urine to flow into toilet (clears urethral commensal bacteria). Pass sterile specimen cup into stream without stopping urine flow; collect 12 to 30 mL of urine (minimum 12 mL for adults; 3–5 mL for pediatric). Discard remaining urine into toilet. SOP-CM-001 §5.2.1
   12–30 mL
4. 4
   SEAL & LABELSeal Container & Label in Patient's Presence: Tighten screw cap securely to prevent leakage. Label container (not lid) immediately with: Full Name, Hospital/Bed No., Date, and Exact Collection Time. SOP-CM-001 §5.2.1
5. 5
   STOP / TIME LIMITPrompt Laboratory Delivery & Temperature Management: Unpreserved urine must be delivered and analyzed within 1 to 2 hours of collection. If analysis is delayed > 2 hours, store refrigerated immediately at 2 to 8 °C (valid up to 6 hours maximum). SOP-CM-001 §5.2.2
   ≤ 2 hours
6. 6
   MEDICAL TECHNOLOGISTEquilibrate Before Analysis: Remove refrigerated urine and allow to stand at room temperature (18 to 25 °C) for 15 to 20 minutes before dipstick or microscopic testing. Cold urine inhibits reagent pad enzymatic reactions, causing false negatives. Mix gently by 5 inversions. SOP-CM-001 §5.2.2
   15–20 min